Most material is
lost after it arrives
A lot released at 99.4% can be worth a good deal less by the time it reaches the bench. Freeze-thaw cycling and moist air do most of the damage, and both are avoidable.
Receipt
Vials travel on phase-change packs rated to hold below 8 °C for 72 hours. Move them to −20 °C within two hours of arrival. A vial that has warmed in transit is not automatically compromised, since lyophilisates are tolerant, but log it.
Before opening
Let the vial reach room temperature before breaking the seal. Opening a cold vial draws in moist air, and water is the main enemy of a lyophilised peptide.
Reconstitution
Run the diluent slowly down the inner wall of the vial rather than directly onto the cake. Swirl; never vortex. Foaming is a sign of denaturation at the air–liquid interface.
Aliquoting
Divide into single-use fractions immediately. Freeze–thaw cycling is the single largest avoidable cause of loss of material in peptide work.
Interactive
Reconstitution calculator
Vial mass
Syringe
- Draw to
- 10.0 units
- Volume
- 0.100 mL
- Concentration
- 2.50 mg/mL
- Draws per vial
- 20
Arithmetic for your vial at the values you entered. Not a protocol and not a dose recommendation. Research use only.
Questions
Asked often
Bacteriostatic water (0.9% benzyl alcohol) for anything withdrawn more than once, sterile water or 0.9% saline for single-use preparations. A small number of lipidated sequences need a little ethanol or DMSO first; the product page states this where it applies.